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目的 分析肝细胞癌（HCC）组织中Glypican-3、HepPar-1、Arginase-1、Ki-67表达水平，并探讨上述指标联合检测对肝HCC病理分化的预测价值。方法 采集中国科学技术大学附属第一医院2023年7月-2024年6月收治的98例HCC患者病理组织，并随机收集70例癌旁组织，采用免疫组化染色法检测磷脂酰肌醇蛋白-3(Glypican-3)、肝细胞抗原（HepPar-1）、精氨酶-1(Arginase-1)、Ki-67表达水平，分析其与临床病理特征的关系，用受试者工作曲线（ROC）分析Glypican-3、HepPar-1、Arginase-1、Ki-67单一指标或联合检测对HCC病理分化的预测价值。结果 与癌旁组织比，HCC癌组织中Glypican-3、HepPar-1、Arginase-1、Ki-67阳性表达率均较高（P<0.05）。Glypican-3、HepPar-1、Arginase-1、Ki-67阳性表达与年龄、性别、肿瘤大小、分化程度均无关（P > 0.05），低分化-未分化HCC患者Glypican-3、HepPar-1、Arginase-1、Ki-67阳性表达均高于高-中分化（P<0.05）。ROC结果显示：Glypican-3、HepPar-1、Arginase-1、Ki-67联合预测HCC病理分化的曲线下面积（AUC）为0.677，高于Glypican-3、HepPar-1、Arginase-1、Ki-67单独检测（0.625、0.600、0.542、0.578）；联合检测特异度为98.70%，均高于各指标单独预测。结论 HCC患者病理分级与Glypican-3、HepPar-1、Arginase-1、Ki-67表达有关，且联合检测对预测HCC病理分化为Ⅲ～Ⅳ级的价值优于单一指标。
Abstract：Objective To analyze the expression levels of Glypican-3, HepPar-1, Arginase-1, and Ki-67 in hepatocellular carcinoma(HCC) tissues, and explore the predictive value of their combined detection for the pathological differentiation of HCC. Methods The pathological tissues of 98 patients with HCC admitted to our hospital were collected from July 2023 to June 2024, and the adjacent tissues of 70 cases were randomly collected. The expression levels of Glypican-3, HepPar-1, Arginase-1, and Ki-67 were detected by immunohistochemical staining, and the associations between these expressions and clinicopathological features were analyzed. The predictive value of Glypican-3, HepPar-1, Arginase-1, Ki-67 and combined detection of the four markers on pathological differentiation of HCC was analyzed by receiver operating characteristic curve(ROC). Results The positive expression rates of Glypican-3, HepPar-1, Arginase-1, and Ki-67 in HCC cancer tissues were higher than those in adjacent tissues(P < 0.05). There was no significant correlation between the positive expression of these markers and age, gender, tumor size, or differentiation grade(P > 0.05). The positive expressions of Glypican-3, HepPar-1, Arginase-1, and Ki-67 were significantly higher in patients with poorly differentiated or undifferentiated HCC than in those with moderate-to-high differentiated HCC(P < 0.05). ROC results indicated that the area under the curve(AUC) for the combined detection of Glypican-3, HepPar-1, Arginase-1, and Ki-67 in predicting HCC pathological differentiation was 0.677, which was higher than that of individual detection(Glypican-3: 0.625; HepPar-1: 0.600; Arginase-1: 0.542; Ki-67: 0.578). The specificity of combined detection was 98.70%, which was superior to that of each individual marker. Conclusion The pathological differentiation of HCC patients is related to the expressions of Glypican-3, HepPar-1, Arginase-1, and Ki-67, and the combined detection of these four markers provides higher predictive value for identifying HCC with pathological differentiation grade Ⅲ～Ⅳ than individual marker detection.
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基本信息:
中图分类号:R735.7
引用信息:
[1]周轶凡,李晓洁,王悦,等.肝细胞癌组织中Glypican-3、HepPar-1、Arginase-1、Ki-67的表达水平在病理分化中的预测价值[J].诊断病理学杂志,2025,32(12):1682-1686.
基金信息:
2021年度安徽省健康委科研项目（AHWJ2021a004）
2025-12-28
2025-12-28